NESC 2570 Study Guide - Final Guide: Dlg4, Cask, Auxilin
27 Jun 2018
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What is the function of synaptobrevin, syntaxin and SNAP25 in
neurotransmission release? What is the roe of Munc 18, NSF and alpha -
SNAP
Syntaxin is present on the membrane as well as SNAP 25 and together
they form the SNARE complex. The formation of the SNARE
complex
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Munc 18 coordinates different SNARE complexes on the same
synaptic vesicles and trigger fusion this way
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NSF unfolds the SNARE complex
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1)
Which protein triggers synaptic vesicle fusion in response to action
potential-evoked calcium influx and does it work mechanistically? Is this
protein required for all forms of neurotransmitter release?
Synaptotanin 1 and 2 which also binds to the SNARE complex
○
Increased affinity to phospholipids which brings the membranes close
together that is energetically unfavourable and causes the fusion of two
membranes
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Syanptotanin 1 and 2 are only required for fast release
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2)
What role do clathrin, dynamin, Hs70, auxilin, PIP kinase 1y, and
snaptojanin have in endocytosis? How is SV endocytosis coupled to SV
exocytosis?
Endocytosis and synapses clathrin lattices forms over the membrane
that needs to be endocytosed
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In order for clathrin to make these lattices, there needs to be AP2 and
AP180 adapter proteins
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Membrane can then attach to the plasma membrane with a fusion stock
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Dynamin forms around the fusion stock and pinches it off - actin may
also have a role in this
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Once vesicles are endocytosed, clathrin cage needs to be disassembled
by Hsc70
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Auxilin binds to clathrin and Hs70 disassembles the protein complex
and allows for the uncoating of vesicles
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PIP2 is generated by a kinase which is only active during neuronal
activity - link to exocytosis
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Phosphotase will take a phosphate group which allows for the
uncoating after the vesicles have endocytosed
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3)
Which proteins are responsible for targeting SVs to the active zone?
Which proteins regulate SV recycling to the active zone?
Actin may either play a facilitory or restrictive role in bringing vesicles
back to the active zone
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Active is actively invovled in the movement of synaptic vesicles
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Vesicles move by fusion and actively move along actin fibers
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Actin can also prevent them from gettting to the active zone - it might
depend on the type of synapse
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4)
What is the function of the active zone cytomatrix? What role do
Munc13, RIM, alpha liprins, CASK neurexins and LAR family protein
tyrosine phsphatase receptors have? What are neuroligins and PSD-95?
5)
Review Questions
October 28, 2015
12:01 PM
Document Summary
What is the roe of munc 18, nsf and alpha - Syntaxin is present on the membrane as well as snap 25 and together they form the snare complex. Munc 18 coordinates different snare complexes on the same synaptic vesicles and trigger fusion this way. Synaptotanin 1 and 2 which also binds to the snare complex. Increased affinity to phospholipids which brings the membranes close together that is energetically unfavourable and causes the fusion of two membranes. Syanptotanin 1 and 2 are only required for fast release. Endocytosis and synapses clathrin lattices forms over the membrane that needs to be endocytosed. In order for clathrin to make these lattices, there needs to be ap2 and. Membrane can then attach to the plasma membrane with a fusion stock. Dynamin forms around the fusion stock and pinches it off - actin may also have a role in this. Once vesicles are endocytosed, clathrin cage needs to be disassembled by hsc70.
