BIOL-2011EL Study Guide - Final Guide: Dna Sequencing, Molecular Probes, Dna Profiling
8 Jan 2018
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Biol 2011 module four: assignment problems: give the dna sequence of the growing chain revealed by the dideoxy sequencing gel below. Give also the sequence of the cloned dna replicated during the sequencing experiment: restriction enzymes are derived fro(cid:373) (cid:271)a(cid:272)teria. Bacterial chromosomes are methylated (the addition or removal of methyl groups) to protect the dna sequence from their own restriction enzymes. The restriction enzymes can cut foreign dna to avoid recombination with the host dna: you are serving on a jury in a murder case in a large city like toronto. The prosecutor has just stated that dna fingerprinting shows that the suspect much have committed the crime. He says that, for the cost reasons, two molecular probes (such as str) were used instead of four, but that the results are just as accurate. No, this evidence alone does not point to the suspect. Dna profiling has been proven to be a conclusive form of evidence.
