1. If you were bitten by a dog, which of the following organisms could be found as a potential pathogen in the resulting wound?
a. Bartonella henselae
b. Afipia spp.
c. Capnocytophaga canimorsus
d. Capnocytophaga ochracae
2. Which of the following tests/characteristics will differentiate Pasteurella and Capnocytophaga?
a. Indole
b. Oxidase
c. Origin of infection
d. Both a and b
3. Your newly acquired kitten can potentially transmit all of the following organisms except
a. Bartonella henselae.
b. Capnocytophaga gingivalis.
c. Pasteurella multocida.
d. Afipia felis.
4. Bartonella spp. cause disease by infecting
a. red blood cells.
b. vascular endothelial cells.
c. the reticuloendothelial system.
d. all of the above.
e. a and b.
5. A homeless 33-year-old male presents to the emergency room with fever, splenomegaly, and bone and joint pain. As the patient changes into a hospital gown, the nurse gathers his clothing and notices the presence of lice. Given this history, which Bartonella species could be a potential causative agent?
a. B. quintana
b. B. henselae
c. B. bacilliformis
d. B. elizabethae
6. Which of the following individuals is most prone to infection with Bartonella henselae?
a. 28-year-old male, visiting the United States from Ecuador, presenting with acute fever and anemia
b. 7-year-old female, who was bitten by a feral kitten, presenting with swollen axillary lymph nodes
c. 20-year-old homeless male with persistent fever and bone, joint, and muscle pain
d. 37-year-old HIV-positive male with fever, chills, anorexia, weight loss. and large skin lesions
7. The human oral isolates of Capnocytophaga can be distinguished from the dog and cat oral isolates by being
a. oxidase positive and catalase positive.
b. oxidase positive and catalase negative.
c. oxidase negative and catalase positive.
d. oxidase negative and catalase negative.
8. Which of the following organisms is/are slow growing and require(s) a carbon dioxide-rich environment?
a. Pasteurella spp.
b. Bartonella spp.
c. Capnocytophaga spp.
d. a and c
e. b and c
9. A physician suspects his patient has an infection due to Bartonella spp. She calls the microbiology department for the specimen of choice for diagnosis. You advise her that the diagnosis is best made using
a. culture of blood.
b. culture of blood vessel tissue.
c. serum for IFA or EIA.
d. a and b.
10. A gram-negative coccobacillus that grows on sheep blood and chocolate agars but not MacConkey agar, is oxidase and catalase positive, and emits a musty odor will be
a. indole positive.
b. a swarming colony.
c. a yellow-orange pigmented colony.
d. normal oral flora of humans.
11. An oxidase and catalase positive, motile, fusiform gram-negative rod is isolated from a dog bite wound. Select the medium and atmosphere where it will grow.
a. sheep blood agar in ambient air.
b. chocolate agar in carbon dioxide.
c. MacConkey agar in carbon dixode.
d. a and b.
12. Which of the following organisms is slow growing and can take up to seven days to exhibit visible growth on subculture even in carbon dioxide?
a. Pasteurella multocida
b. Capnocytophaga canimorsus
c. Bartonella henselae
d. Both a and b
e. Both b and c
13. A 49-year-old male with AIDS presents with fever, sepsis, and disseminated intravascular coagulation. Blood cultures drawn demonstrate a fusiform gram-negative rod after five days of incubation. The organism grows on sheep blood and chocolate agars after 48 hours incubation in a carbon dioxide atmosphere. It proves to be oxidase and catalase positive. What information in the patient history could help correlate the source of this organism with its identification?
a. Recent travel history
b. History of alcoholism or homelessness
c. History of exposure to animals
d. Recent dental manipulation
14. A veterinarian presents with a purulent hand wound. The direct Gram stain reveals a gram-negative coccobacillus. On culture, the organism grows on sheep blood and chocolate agars in CO2 but not on MacConkey agar. Which of the following profiles can be used to presumptively identify this organism?
a. A yellow, spreading colony that is oxidase and catalase positive and indole negative
b. A flat gray colony that is oxidase, catalase, and indole positive
c. A small, dry embedded colony that is oxidase, catalase, and indole negative
d. An orange spreading colony that is oxidase positive, catalase negative, and indole positive
15. A previously healthy 5-year-old female presents with swollen axillary lymph nodes and fever. The physician notes multiple scratches and papules on her hands and arms. The mother reveals they had adopted a new kitten about two weeks ago, and it had scratched the child numerous times. She said she cleaned and treated the scratches with hydrogen peroxide immediately after each occurrence. Using the information provided, this infectious disease state requires
a. No antibiotic therapy because the infection will resolve on its own
b. Confirmation using a biopsy and culture of the lymph nodes is required
c. Antibiotic therapy for adequate treatment
d. A culture of the kitten to determine the specific infective organism
