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BIOL 200 Final: Reverse transcription of the retroviral genome

Canada

Molecular Biology

Biology

Thomas Bureau

McGill University

<p></p>
<div> Discuss the principle of reverse transcription PCR.</div>

<p><p>Provine a detailed discussion of central dogma and reverse transcription (20)</p></p>

<p><span class="Apple-style-span c1">Reverse transcription ofretroviral mRNA is a baroque process with multiple primers andtemplate 'jumps'. Why is this complexity necessary?</span></p> <table class="c2"><tbody> <tr> <td><table class="c2"><tbody><tr><td></td></tr></tbody></table></td> <td>A. Copying RNA into DNA is inefficient</td> <td></td> </tr> <tr> <td><table class="c2"><tbody><tr><td></td></tr></tbody></table></td> <td>B. Because the viral mRNA is not translated when it enters thehost cell</td> <td></td> </tr> <tr> <td><table class="c2"><tbody><tr><td></td></tr></tbody></table></td> <td>C. Because the mRNA is not a complete copy of the genome, theLTRs must be regenerated</td> <td></td> </tr> <tr> <td><table class="c2"><tbody><tr><td></td></tr></tbody></table></td> <td>D. The primer for reverse transcription is a tRNA</td> <td></td> </tr> <tr> <td><table class="c2"><tbody><tr><td></td></tr></tbody></table></td> <td>E. DNA synthesis is very slow</td> </tr> </tbody></table>

BIOL 200 Final: Reverse transcription of the retroviral genome

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