BIOL 200 Study Guide - Restriction Fragment Length Polymorphism, T7 Rna Polymerase, Rna Polymerase Iii

• School: McGill University
• Department: Biology (Sci)
• Course: BIOL 200
• Professor: Richard Roy

Document Summary

Lac promoter-> t7 rna polymerase -> t7 promoter-> recombinant gene expression neor (selection marker) Rna polymerase i (ribosomal precursor rna genes in nucleolus) Upstream element + upstream activating factor (uaf) Core element + trimeric core factor (+tbp) Insensitive to alpha amatin; no need for atp. Rna polymerase ii (protein encoding genes; rnas for splicing; small non-coding functional rnas) Rna polymerase iii (ribosomal rna genes not in nucleolus; trnas; small stable rnas including u6) Intermediate sensitivity to alpha amatin; no need for atp. Tfiiic (with tfiiia in 5s), tfiiib, rna pol iii (+tbp) Xeroderma pigmentosum (ner unable to repair thymidine dimers) Heptapeptide repeat in large subunit of rna pol ii (+capping, splicing, polyadenylating factors) Maturation of nacent transcript (capping and methylation on 5") Spliceosome: 5 snrnps (each consist of snrna + 6-10 proteins) Group ii introns (some rrna and trna genes in mitochondria and chloroplasts) Cross-exon recognition complex (u2, u2af, sr proteins, u1) Fast phase (pap + pabp ii- 200a.