BIOL 301 Study Guide - Final Guide: Inbreeding, Transposable Element, Ubiquitin
10 Apr 2018
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Collection of mutants for every gene in arabidopsis. Transposons in drosophila tend to jump to nearby sequences. Use specific primers for end of inserted dna and random primers. Cross with mapping line, first determining chromosome, then determining location on chromosome. Create heterozygote for linked genes, then cross to tester line (homo recessive) Pcr-based: migration with more repeats (heterozygote has two bands) Inserted dna acts as tag for genes involved. Only one cross to test whole genome (since inbred lab strains have many dispersed polymorphisms) Forward genetics via point mutations (truncation, loss of key amino acids) Knock out gene to see effects on protein. If you use a taq polymerase with no modifications, it is error-prone. Identify a small number of candidate genes (find location in the genome of mutated gene) Run a known genotype sample of each strain: both homozygous and the heterozygous marker beside your samples to compare the size.
