BIOL 301 Study Guide - Midterm Guide: Escherichia Coli, Microbiological Culture, Tetracycline
Document Summary
Gene expression analysis, dna chips, northern blot, rt-pcr. Promoter-reporter gene fusion, gene expression analysis, reverse genetics, rnai. Random mutations, select phenotype of interest, find gene responsible. Identifying and isolating mutants that possess a phenotype of interest. Identifying the function (phenotype) of a gene of interest. Locating a gene of interest in the genome by identifying nearby landmarks by determining linkage using recombination frequency. Can visualize the phenotype by running marker dna on a gel. Dna agarose gel, smaller dna fragment, higher % agarose. Rna, oligonucleotides & small pcr products agarose/acrylamide or. Polyacrylamide gels, sds used to remove charge component (gives negative charge) Used for positional cloning of a gene for which you have a mutant phenotype. Cross homozygous mutant from f2 generation to a marker line. Insert tag into a gene to disrupt it. Tail-pcr can be used to amplify flanking sequences.
