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BIOL200 Review - Names.docx

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McGill University
Biology (Sci)
BIOL 200
Richard Roy

Lecture16- Molecular Genetics EcoRI Complementary DNA (cDNA) Reverse transcriptase Poly dG adapter (annealed to 3’) Poly dC primer Alkali (remove RNA) RNAase H (remove RNA) Lac promoter-> T7 RNA polymerase -> T7 promoter-> recombinant gene expression neo (selection marker) Green Fluorescnt Protein(GFP) Nylon support Nitrocellulose support Polynucleotide kinase (PNK) Restriction fragment length polymorphisms (RFLPs) Breast cancer related 1 (BRCA1) Lecture17- Eukaryotic Transcription 1 RNA Polymerase I (ribosomal precursor RNA genes in nucleolus) Insensitive to alpha amatin; no need for ATP Promoter:  UPSTREAM element + UPSTREAM activating factor (UAF)  CORE element + trimeric CORE factor (+TBP) RNA Polymerase II (protein encoding genes; RNAs for splicing; small non-coding functional RNAs) Highly sensitive to alpha amatin; CTD RNA Polymerase III (ribosomal RNA genes not in nucleolus; tRNAs; small stable RNAs including U6) Intermediate sensitivity to alpha amatin; no need for ATP Promoter (within transcribed region):  Internal promoter element-A Box- tRNA  Internal promoter element-B Box- tRNA  Internal promoter element-C Box- 5S  TFIIIC (with TFIIIA in 5S), TFIIIB, RNA Pol III (+tbp) Carboxy terminal domain(CTD) TATA box TATA-box Binding Protein (TBP) TFIID (+TBP) TFIIB TFIIF+RNA Pol II TFIIE TFIIH (possesses helicase and kinase activity) Couples transcription and repair Holoenzyme Xeroderma pigmentosum (NER unable to repair Thymidine dimers) Lecture18- Eukaryotic Transcription 2 GAL 4 UAS-gal4 System Antennapedia Zinc-finger DNA binding domains  C H 2 2  C4types  C6types Leucine zipper proteins Helix-Loop-Helix proteins MADS box TFs  APETALA1  APETALA3  PISTILATA  AGAMOUS  SEPALLATA3 (accessory) Lecture19- RNA Processing 1 Heptapeptide repeat in large subunit of RNA pol II (+capping, splicing, polyadenylating factors) S5-P S2-P Nascent transcript Maturation of nacent transcript (capping and methylation on 5’) 7’methylguanylate cap (5’-5’ linkage) GU---A—Pyrimidine rich region---AG (GU-AG conserved border region) Transesterification reactions Lariat intron Spliceosome: 5 snRNPs (each consist of snRNA + 6-10 proteins) Debranching enzyme Group I introns (rRNA genes of protozoans) Self-splicing Group II introns (some rRNA and tRNA genes in mitochondria and chloroplasts) Self-splicing Lecture20- RNA Processing 2 U2AF65 U2AF35 Exonic splicing enhancers SR proteins with RPM domains Cross-exon recognition complex (U2, U2AF, SR proteins, U1) Sex Lethal protein (Sxl) Transformer protein (Tra) Rbp1 SR protein Tra 2 SR protein Double sex protein (Dsx) Presumptive exons AUUUA in 3’UTR Destabilizing motif for RNAs Polyadenylation Not in histones  Slow phase (PAP) -12A  Fast phase (PAP + PABP II- 200A AAUAAA xxxxx G/U sequence recognition by cleavage and polyadenylation factors AAUAAA Polyadenylation signal Cleavage factor I and II (CFI and CFII) Cleavage and polyadenylation specificity factor (CPSF) Poly A polymerase (PAP) PABP II RNA editing: Deaminating enzyme (A->I; C->U) Apolipoprotein B Lecture21- Nucleo-Cytopl Transport Hydrophobic FG domains FG repeats FG nucleoporins Nuclear Pore Complex (NPC) Nuclear Localization Signal (NLS) Lysine, arginine rich Nuclear Export Signal (NES) Leucine rich Pyruvate kinase Ran GTPase Importin alpha Binds NLS Importin beta Binds FG repeats GTPase acceleration function protin (GAP) Guanine exchange factor (GEF) Exportin 1 For regular cargo Tri-molecular cargo complex Exportin t For tRNAs mRNA exporter (Tap+ Nxt1) For mRNAs; Ran-independent mRNP remodeling Insect polytene chromosomes Balbi
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