BIOL 301 Study Guide - Midterm Guide: Embryonic Stem Cell, Northern Blot, Housekeeping Gene
2 Apr 2013
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Question 1 eyeless tf of regulation of eye development in drosophila. You constructed 5 different rnai constructs that target different portions of eyeless mrna. These constructs are expressed in the eye using an eye specific promoter. Only three constructs show no eyes phenotype while two others produced smaller but visible eyes. Rnai caveats some are specific and some might not work. Explain this discrepancy and how would you test it (not make new rnai construct) Hypothesis: maybe some constructs work better than others. No signal from null mutant know gene is not expressed. Housekeeping gene** most important control because comparing expression levels. Question 2 differentiated cells can be reprogrammed to an embryonic-like state by transfer of nuclear contents into oocytes or by fusion with embryonic stem cells. Little is known about factors that induce this reprogramming. In order to address this question, you would like to know which genes are specifically expressed or repressed in these reprogrammed cells.
