Transmission electron microscope (shows the peroxisome in detail)
Fluorescent microscopy: can see all peroxisomes as small dots: cannot see structure of
Peroxisomal transport requires PTS1
What do all protein transport pathways have in common?
1. Signal sequence on PRO
2. Receptor for seq
3. Translocation channel
4. ATP hydrolysis
5. A way of getting PRO to different locations into diff locations in organelle
PTS1 (peptide) is necessary for import of PRO into peroxisome. If the peptide is
modified transport will not occur. GFP stays in cytosol.
PTS1 is sufficient (all that we need) for PRO import into peroxisome. When PTS1
attaches to GFP, GFP moves into peroxisome.
Pex 12 is necessary for transport into the lumen of peroxisome but not the membrane.
Pex12 is required for transport into lumen but not membrane
Pex3 gene is necessary for transport into membrane, but since no membrane PRO are
here nothing can get into the lumen.
Electron microscopy is on a whole organelle level.
Strong fluorescence is found in targeted location
Are PRO targeted to the mitochondria cotranslationally or posttranslationally?
Hyp: fully translated PRO can be transported into mitochondria.
If GFP PRO go