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BIO review.docx

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Department
Biology
Course
BIOLOGY 2B03
Professor
Kim Dej
Semester
Fall

Description
Slide 1 Transmission electron microscope (shows the peroxisome in detail)   Fluorescent microscopy: can see all peroxisomes as small dots: cannot see structure of  peroxisomes Slide 2 Peroxisomal transport requires PTS1 Slide 3 What do all protein transport pathways have in common? 1. Signal sequence on PRO 2. Receptor for seq 3. Translocation channel 4. ATP hydrolysis 5. A way of getting PRO to different locations into diff locations in organelle PTS1 (peptide) is necessary for import of PRO into peroxisome. If the peptide is  modified transport will not occur. GFP stays in cytosol. PTS1 is sufficient (all that we need) for PRO import into peroxisome. When PTS1  attaches to GFP, GFP moves into peroxisome. Pex 12 is necessary for transport into the lumen of peroxisome but not the membrane.  Pex12 is required for transport into lumen but not membrane Pex3 gene is necessary for transport into membrane, but since no membrane PRO are  here nothing can get into the lumen. Electron microscopy is on a whole organelle level. Strong fluorescence is found in targeted location Are PRO targeted to the mitochondria co­translationally or post­translationally? Hyp: fully translated PRO can be transported into mitochondria. If GFP PRO go
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