BIOLOGY 2C03 Study Guide - Midterm Guide: Sickle-Cell Disease, Long Term Ecological Research Network, Statistical Hypothesis Testing
14 Oct 2017
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Document Summary
Snp - single nucleotide polymorphism, is a single nucleotide di erence approx. 10 m in human genome and mostly present in non-coding area. Southern blotting restriction enzyme cut dna into small fragments gel electrophoresis of the dna fragments and then transferred to nitrocellulose membrane membrane exposed to uorescent labelled-hybridization probes and pattern visualized in lm. Dna microarray consists of series of thousands of spots of aso used as dna probe. 2 probes - one for wt and 1 for mutation, both means yellow (heterozygous) micro satellites also known as short tandem repeats (strs) Pcr ampli cation and gel electrophoresis detect micro satellites size of fragment depends on # of repeats. Discovering genes implicated in disease humans cannot be studied through controlled mating to create genetic maps - unethical there are 3 approaches in doing this: linkage analysis association studies genome sequencing.
