LIFESCI 2A03 Study Guide - Midterm Guide: Blastema, Embryonic Stem Cell, Cellular Differentiation
13 Oct 2013
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Scientific method: define/identify problem, form hypothesis, test hypothesis, organize and analyze data, experiments; do observations support hypothesis? (if no, perform new experiments, communicate results. Case study human chromosome count: theophilus painter, 1923 reported 48 chromosomes in human cell by performing serial sections of testicular cells in metaphase and counting chromosomes. Problems chromosomes clumped together, curled in and out of focal plane, Repeatability other researchers confirmed count using same techniques; many people took result as authoritative. Can see entire cell at once; spread out metaphase chromosomes. Ideograms used to line up chromosomes (largest to the left: karyotyping now used to line up chromosomes; fluorescently stained based on genes present. Hypothesis proposal or explanation that makes a prediction about the result of intervention and can account for observations; application of the scientific method allows testing of hypothesis. Inductive logic inductive generalizations summarize a set of observations and serve to provide a prediction about unseen events.
