LIFESCI 2A03 Study Guide - Midterm Guide: Embryoid Body, Thymidine, Blastocyst

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Module #6: planaria - stem cells and regeneration: technique 1: labeling cells that are actively diving (neoblasts), a: labeling with brdu brdu is analogous to thymidine. Assaying directly for the presence of mrnas from transcription. A probe is a small sequence of dna that is complementary to a target sequence. Since it is complementary, it will hybridize in a base pair specific manner: technique 3: manipulating gene function, rna interface, gene is silenced post-transcriptionally. mrnas are destroyed. This allows the researcher to identify a subset of the genes that are required for a specific task, such as regeneration: neoblasts can be identified by, morphology of the cells. Stem cells are characterized by having a large nucleus to cytoplasm ration: brdu labeling labeling s-phase or dna replication, antibody to histone h3 identifying metaphase of mitosis during which chromosomes condense. We can then conclude that the mitotically dividing stem cells are required for regeneration: neoblasts are required for regeneration, a: un-irradiated fragment.

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