LIFESCI 2A03 Study Guide - Midterm Guide: Asymmetric Cell Division, Adult Stem Cell, Null Hypothesis
26 Nov 2013
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Adult stem cells cells that can divide to create two cells (another stem cell or differentiated cell) Symmetric cell division two identical daughter cells are created (both stem cells) Stem cell niche microenvironment that maintains stem cell identity. Gsc remains in niche; retains stem cell identity. Cellular marker used to identify gsc use antibody to proteins within fusome/spectrosome (only found in germ line cells) Intrinsic expression of bam, differentiation- promoting gene. Cb require expression of two genes (bam (bag of marbles) and bgcn (benign gonial cell neoplasm)) needs to differentiate. Extrinsic gsc in niche, cb out of niche. Can visualize bam gene by fusing bam promoter with gfp coding sequence (bamp- gfp) Promoter specific dna sequences required for binding tfs (that recruit rna polymerase) and rna polymerase. Experiments: remove bam gene function germ cells do not develop; all cbs, no cysts or gscs. Info bam only present in cb; outside of niche.
