MOLBIOL 3B03- Midterm Exam Guide - Comprehensive Notes for the exam ( 13 pages long!)
6 Oct 2017
School
Department
Course
Professor
Document Summary
Protein production depends: mrna stability, protein stability, transcription rate. Transcription requires: dna template + dnap/rnap polymerase + promoters + transcription factors. Cis elements- non-coding dna that affect s the transcription of genes on the same dna molecule. Trans elements- non-coding dna that affects the transcription of genes on another dna molecule. Transcription depends on cell, cell types, cell stage and genes. Control of transcription initiation is the most impt step. P: couple transcription and translation can occur; cannot occur in. 35 region = ttgaca: secures rnap to promoter, pribnow box (-10 region) = tataat, where transcription begins and initiates dna melting, some variation in sequence => broad specificity. : two subunits; interact with regulatory sequences to control initiation frequency of promoter. 70 : initiation factor; recognizes promoter region, increases rnap affinity to promoter and decreases non-specific: released after polymerization ~10 rntps binding. Activated: presence of lactose binds to repressor = inactive, so it cannot bind to operator.
