CHEM 412 Midterm: CHEM 413 Midterm
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Name: ______________________: (3 pts) in the laboratory, we denatured a protein with a number of different agents. Which and how: (3 pts) a reversible inhibitor acts by binding only to free enzyme. Why: (5 pts) ok, i just simplified this problem dramatically. It is possible to have mm type kinetics without saturating the enzyme. Derive the rate equation for this simple system and express it in the form of the mm equation, indicating what the apparent kcat and km terms are. k1. E: (3 pts) here s a fun game of circle the pretty pictures! It turns out that there is another way to do this that is under investigation by steve withers at the university of british. Take a look at the proposed mechanism below, and address the questions that follow. 1 yip, v. l. y. and withers, s. g. (2006) mechanistic analysis of the unusual redox-elimination sequence. Employed by thermotoga maritime bglt: a 6-phospho-b-glucosidase from glycoside hydrolase family.
