CHEM 412 Final: CHEM 413 Final Exam 2006
Document Summary
I know you haven"t slept a wink last night, but don"t panic: the following is the catalytic cycle of pyruvate decarboxylase, a thiamine dependent decarboxylase that we discussed in class. Some recent work has been published on this system, increasing the overall level of joy we"ll have with the system. This problem is similar to one last year, so don"t panic: (6 pts) ok, this is a bit long, but shouldn"t be hard since i"ve given you all of the key intermediates. Show the full arrow-pushing mechanism for this transformation. Using this critical piece of data postulate which step is rate determining and explain, in detail, why (there is more than one possible correct answer). Please ignore all of the extra space; it is to make the next page easier to write. 1 transition-state responses to amino acid perturbations in yeast pyruvate decarboxylase: a carbon kinetic isotope effect study l. chen, y. yuan and w. p. huskey j. phys.
