NSCI 323 Study Guide - Final Guide: Synaptic Plasticity, Fear Conditioning, Amygdala

• School: Queen's University
• Department: Anatomy and Cell Biology
• Course: NSCI 323
• Professor: Rose, Paul Kenneth, Dumont, Eric C

Document Summary

Postsynaptic receptor trafficking underlying a form of associative learning. Wanted to see intracellular changes associated with learning and memory (ltp), esp. More cellular level of phenomena, less correlation front, but rather provided more causative mechanism for memory. Prior, knew ampa trafficking was related to learning, but no one ever causally linked the two (a) Cells were infected with virus, which is identifiable with the green fluorescent proten (gfp) Glur1-gfp: normal glur1 protein with tag normal plasticity ( plasticity tag ) Glurc-c-tail-gfp: last c-terminal of glur1 subunit with tag. No incorporation of glur1 receptors, blocks synaptic plasticity. Images of coronal section right hemisphere including amygdala. Gfp-expressing cells shown in lateral amygdala (dotted line) (d) and (e) Shows neurons expressing gfp (top arrow) (f) Infected and non-infected neuron stimulated with current injections. The two recordings are the same; there was no effect on signal transmission no effect on electrophysiological properties.