BCHM 218 Study Guide - Final Guide: Meiosis, Deinococcus Radiodurans, Spo11
13 Oct 2016
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G2: assay for activity chromatid pair via centromere. S two identical chromosomes remain together as a sister. M duplicated chormosomes separate and cell divides into two. Go quiescent can last hours or lifetime. Measurement of the product of the reaction being catalyzed daughters (each 2n) by cytokinesis. Add radioactivity or fluorescently labeled dntp: detection of dna polymer. Use + charged filter that binds to - charged polynucleotide backbone while unincorporated nucleotides will pass through. Contains labeled dntps: the more radioactivity caught, the more the polymer is doing. When you don(cid:495)t know anything about the enzyme in dna. First need to isolate from other enzymes replication: ion exchange chromatography, size exclusion chromatography. Demonstrated that pol i received instructions on what nucleotides to incorporate from an existing template (parent dna: dna replication is semi-conservative. Used experiment using 14n and 15n having different weights to determine: origins of replication (many) Reads (cid:885)(cid:495) to 5(cid:495), but copies 5(cid:495) to (cid:885)(cid:495)
