BCH 361 Study Guide - Quiz Guide: Glycogen, Horse Length, Alcohol Dehydrogenase
18 Sep 2018
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Wavelength is missing from the y-axis label in figure 1. Question 2: yes, yes, no d)yes, yes, yes, no, yes, no, yes. It measures the activity by using a spectrophotometer at 340 nm. The extinction coefficient of nadh is given as 6. 2 nm-1 cm-1. Nadph is measured by noting the change in absorbance at 340 nm. 5. 0*10^-5 mol/1. 0*10^-3 l= 0. 05 mol/l *1000 = 50 mm ethanol. 60 mm= 0. 06 m c1*v1=c2*v2 c2= (5. 0*10^-5 l) (0. 06 m)/ (1. 0*10^03 l) 20 microliters= 0. 02 ml 0. 1 g/ml= 2. 010^-3 g*1000= 2mg liver extract/ml. Liver homogenate (0. 1 g/ml prepared in 50 mm tris, ph 7. 5, mm ddt) was assayed in 0. 4 m glycine ph 9. 5, 50 mm ethanol and 3 mm nad. 2 mg of liver was added to the assay. The reaction activity was determined by observing change in absorbance at 340 nm over a duration of. The beer-lambert law was used to calculate the reaction rate.
