BCH 361 Study Guide - Quiz Guide: Glycogen, Horse Length, Alcohol Dehydrogenase
112 views3 pages
Learning about Captions
Wavelength is missing from the y-axis label in figure 1.
How does this experiment measure the activity of alcohol dehydrogenase?
It measures the activity by using a spectrophotometer at 340 nm. The beer-lambert equation
A=Elc. The extinction coefficient of NADH is given as 6.2 nM-1 cm-1.
What reaction with what?
Ethanol + NAD reacted with alcohol dehydrogenase.
What was the product?
What was the relationship between the product and the activity of alcohol dehydrogenase?
NADPH is measured by noting the change in absorbance at 340 nm.
What was the final glycine concentration if 0.5 mL of 0.8 M glycine is used?
(0.8) *(5.0*10^-4)/(1.0*10^-3)= 0.4 M
What are the final NAD and ethanol concentrations?
5.0*10^-5 mol/1.0*10^-3 L= 0.05 mol/L *1000 = 50 mM Ethanol
50 microliters= 5.0*10^-5
60 mM= 0.06 M
c2= (5.0*10^-5 L) (0.06 M)/ (1.0*10^03 L)
= 3.0 *10^-3 *1000 3mM NAD
What is the final concentration (g/mL) of the liver homogenate?
400 mg/4 mL= 100 mg/ml= 0.1 g/ml