BLG 307 Study Guide - Centrifugation, Thermus Aquaticus, Immunofluorescence

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Field of science dedicated to the study of dna on a cellular scale. 5 general steps for cloning of dna: cut dna at precise location, selection of a small dna piece, capable of self, joining the 2 fragments covalently, identification of host cell that contains recomb dna replicating. The ability of a single stranded nucleic acids to for a duplex. Heating causes the strands of a dna duplex to separate (denaturing: requires temps around 90 c. Complementary ss can be renatured when cooled down: typically reducing heat or after ph change. Commonly made in lab by solid-phase chemical synthesis. Can be used as a probe or in pcr. Theoretical melting temperature of a duplex nucleic acid segment into separate strands. Temp at which the dna is denatured. Depends on several parameters including sequence composition content. Relationship between dna mt and guanine +cytosine: more gc=higher tm (related to h-bonding)