MBB 222 Study Guide - Final Guide: Rna Polymerase Ii, Cistron, Transcription Bubble

• School: Simon Fraser University
• Department: Molec Biol & Biochem
• Course: MBB 222
• Professor: Edgar Young

Document Summary

Mbb222 exam vocab definitions: bacterial transcription initiation and eukaryotic. U1-08 must promoter -10 vs. -35 vs. up region (with numbering convention, The -10 and -35 positions are important interaction sites for the sigma70 subunit. Numbering convention: dna base pairs that precede the rna start site are given negative numbers and base pairs corresponding to the beginning of the. Certain nucleotides that are particularly common at each position form a consensus sequence. Ex. ) the consensus sequence at the -10 region is (5")tataat(3") and. U1-08 load cistron, polycistronic at the -35 region it is (5")ttgaca(3") . A cistron is a section of an rna molecule that codes for a specific polypeptide in protein synthesis. Many bacterial mrnas are polycistronic, which means that they have multiple genes on a single transcript. An operon is the gene cluster, promoter and additional sequences that function together in regulation of the gene expression. Many operons include 2-6 genes transcribed as a unit, some contain 20+