[MBB 222] - Final Exam Guide - Ultimate 29 pages long Study Guide!

• School: Simon Fraser University
• Department: Molec Biol & Biochem
• Course: MBB 222
• Professor: Edgar Young

Document Summary

Know correct spelling of full length names. Know 3 letter codes for amino acids (pro = proline) Be able to write the protein sequence given the codon table. Know how they interact, their properties (from functional groups) Dnaa: recognition of ori sequence, opens duplex at specific sites. Dam methylase: methylates gatc sequences at oric. Dnap i: filling gaps in sequences and excision of rna primers. Dna gyrase: helps relieve tension on the strand (reduces supercoiling) Be able to read a sequence with promoters, operators. Operators and activator binding sites bind to repressors and activator proteins (respectively) turning promoters on and off. These regulatory sequences are usually near the promoter. Effectors either decrease or increase affinity of the regulatory protein for dna. Inducers: induce gene expression by decreasing the affinity of the repressor to the operator upon binding. Corepressors: increases the affinity of the repressor for the operator.