MBB 222 Study Guide - Final Guide: Whole Genome Sequencing, Chromosome, Polymerization
17 Dec 2016
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Document Summary
Given a known genomic library and an unknown cdna library, we can identify what subset of the genes in the genome are present in the cdna library. Dna microarrays allow for the rapid and simultaneous screening of thousands of genes and provides a snapshot of all the genes in an organism. Microarrays can reveal expression patterns of genes that change in response to cellular stimuli, development stage or conditions. Unknown cdna molecules are attached with dye in solution. First, dna segments from genes of known sequence are amplified by. Then, nanomolar quantities of the dna solution are deposited into an array, with each spot containing sequences derived from a particular gene. The single stranded dna is placed into a well so it can hybridize. Hybridization: when the cdna added will bind to the known dna clones in the wells as they are complementary. This is why the dna added must be ssdna (single stranded) so they can undergo hybridization.
