MBB 222 Study Guide - Final Guide: Ampicillin, Polyadenylation, Beta-Galactosidase

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Mbb222 exam vocab definitions: restriction cleavage and ligation, selection. U4-06 first restriction endonuclease (restriction enzyme), type ii. Restriction endonucleases recognize and cleave dna at specific sequences. Type ii variants are simpler than type i and iii, as it requires no atp and catalyzes the hydrolytic cleavage of particular phosphodiester bonds in dna within the recognition sequence itself. Restriction sites are palindromic , meaning that their complementary strand as the identical sequence in the 5"-3" direction. U4-06 must recognition sequence vs. cleavage site. Recognition sequences are palindromic sequences recognized by restriction endonucleases to cleave at (usually 4-6 bp long) Cleavage sites are the location where the cuts are made by restriction endonucleases that can either be staggered cuts or blunt ends. Cleavage sites are much smaller, while the recognition sequences are bigger as they are a sequence. U4-06 first staggered cut (cohesive / sticky end) vs. blunt end.