MBB 222 Study Guide - Final Guide: Pyrimidine, Hypochromic Anemia, Purine

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U4-01 must duplex / double-stranded vs. single-stranded. Double stranded refers to a dna (or rna) molecule that consists of 2 strands together. Single stranded refers to one dna/rna strand. U4-01 first dna hybridization / annealing / renaturation vs. denaturation / melting. Dna hybridization = the formation of the double helix structure from a single stranded nucleic acid. The double helix can be a duplex or double stranded. Annealing and renaturation mean the same thing (best to say formation of duplex) When you are referring to both dna, both rna or one of each. Base-pairing in the final duplex may not be perfect (not totally complementary) The 2 single strands may come from the same source or form different ones. Denaturation or melting or strand separation = the opposite of duplex formation and refers to the unwinding and breaking of the hydrogen bonds in the duplex.

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