MBB 222 Study Guide - Final Guide: Allele, Reverse Transcriptase, Combined Dna Index System

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Mbb222 exam vocab definitions: dna sequencing, pcr and forensics. The process of working out the exact order of the 4 bases in a strand of dna. Start with denaturing the double stranded dna molecule to be sequenced via naoh solution (will separate the strands) Solution containing the single strand of dna is mixed with: Radioactively labeled dna primer (complementary to the 3" end of the strand to be sequenced as this will allow proper polymerization in the 5"-3" direction) 4 types of deoxynucleoside 5"-triphosphates (datp, dgtp, dctp, dttp) A small quantity of a specific dideoxynucleoside triphosphate (ddntps) Dna pol will sometimes use these ddntps causing it to stop polymerization, causing many fragments to be made. 4 solutions are used with each specific ddntp. After the 4 reactions are completed, gel electrophoresis is done to each reaction mixture to produce 4 lanes separating fragments based on their size (fragments travelling the farthest are the smallest)

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