MBB 222 Study Guide - Final Guide: Amphoterism, Isoelectric Point, Zwitterion

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Ampholyte = contains 2 ionizable groups, thus there are both acidic and basic pka values. Exist as zwitterions in solution of neutral ph. Polyampholyte = large molecules (ex. proteins) that can have many acidic and basic groups. When an amino acid lacking an ionizable r group is dissolved in water at neutral ph, it exists as a zwitterion, which can act either as a acid or base. Meaning they have 0 net charge as they have both a (+) and (-) charge. The ph in which the protein"s net charge is zero. The isoelectric point is simple for a molecule with only 2 ionizable groups, it is simply the average of the 2 pka values. Pka1 = 2. 34, pka2 = 9. 60, the 1st is for -cooh and 2nd for -nh3. U6-05 first average net charge (e. g. fractional charge) Step 1: identify all the ionizable groups in the peptide. Step 2: gather the pka data of each ionizable group.

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