MBB 222 Study Guide - Final Guide: Specific Activity, X-Ray Crystallography, Statistical Hypothesis Testing
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Mbb222 exam vocab definitions: specific activity and structure determination. U6-03 first purification scheme, purification steps, purification table. A purification scheme consists of several purification steps done to a sample of protein. These steps can be organized and analyzed via a purification table that displays the quantity of proteins present, the activity and the purification level. The goal of purification in enzymes is to maximize specific activity. Refers to the sample of a crude mixture of unpurified proteins being observed. Preparation for purification refers to making sure it is mixed with the right buffer and measuring the correct amount of protein etc. The assay is a test for a particular unique identifying characteristic of a protein. Therefore a positive result on the assay indicates the protein is present. A more specific assay produces a more effective purification. U6-03 first activity (of an enzyme) vs. specific activity. Activity = refers to the total units of enzyme in solution.