MBB 222 Study Guide - Final Guide: Polyacrylamide Gel Electrophoresis, Sodium Dodecyl Sulfate, Gel Electrophoresis Of Proteins

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Mbb222 exam vocab definitions: protein electrophoresis and protein sequencing. This is the relative migration of a protein on an sds gel and is related to its relative molecular mass. Electrophoresis is distinct from gel filtration because all molecules regardless of their size are forced to move through the same matrix. Is a factor in the velocity or migration of a protein. F depends on the mass and shape of the migrating molecule and the viscosity of the medium. Sds-page = sodium dodecyl sulfate polyacrylamide gel electrophoresis. Separates and stains the proteins present at each step of purification. Small sample from each fraction/step is mixed with detergent (sds) and heated. This unfolds the proteins and coats them with a negative charge. Reducing agent is included to reduce disulfide bonds. Then the denatured samples are applied to wells in a gel, where a voltage is applied across it.