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BIOL 2000 Study Guide - Midterm Guide: Escherichia Coli, Lytic Cycle, Auxotrophy

Course Code
BIOL 2000
Joseph Rasmussen
Study Guide

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Midterm #3
1. A single, visible isolated colony of bacteria growing on an agar plate
a. Approx. 100 bacterial cells
b. A population of auxotrophic bacteria, since prototrophic bacteria can’t
be grown on an agar plate.
c. A mixture of several variants, die to differences in DNA sequence, but
which all belong to the same species
d. A population of bacteria derived by multiple cell divisions starting
from a single cell.
e. None of the above
2. During the phage lytic cycle, which one of the following events would not
a. Integration of the phage genome into the bacterial genome
b. Lysis of the bacterial cell to release new phage progeny
c. Fragmentation of the bacterial cell’s chromosomal DNA
d. Synthesis of phage proteins
3. An E. Coli cell has the following genotype: bio- leu- arg- It is trasnfermed with
DNA from a strain that is bio+leu+arg+. Which of the following genomic
arrangements is consitant with these reults?
19% bio+ 8%
3% bio+ 7 % arg+
a. Bio arg leu
b. Arg bio leu
c. Arg bio leu
d. Bio leu arg
4. IN E. Coli DnaA protein:
a. Is the helicase used to separate the template strands during
b. Coats the newly synthesized DNA strands at the replication fork to
prevent them from base-pairing
c. Binds conserved sequence box elements located at the chromoal
origin of DNA replciaiton
d. Removes supercoilds from the DNA
e. Synthesizes the leading strand during DNA replcaition
5. The Herhsey-Chase experiment was an mportant experiement that
demonstrated that DNA was heritable marterial. The researchers used a
virus that had either its DNA or protein componenets specifically
radioactively labeled. Which one fo the following statements aout this
experiment is false.
a. Researchers used a bacteriophage to infect E Coli cells
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