BIOCH499A Study Guide - Hydrogenation, Homogeneous Catalysis, Heterogeneous Catalysis
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Give it a try under simulated conditions: 45 min, closed book, etc. Can"t measure [es] k1[e][s] k 1 [es] + k2 [es] (steady state approx) So the rate is k1[e][s] k 1 + k2. You are expected to know how to arrive at this result. The next page shows how to obtain the final result. You are not responsible for the derivation but you should know the page after that (p. 5) on how to use the final equation and the plot on p. 6. Or on one line e + s es e + products. Unfortunately, it is not convenient to measure the concentration of the free enzyme. Instead, the total enzyme concentration [eo] is measured. (it includes the free and bound enzyme). [es] = k1[e][s] k 1 + k2 (k 1 + k2) [es] = k1[eo][s] k1[es][s] (k 1 + k2) [es] + k1[es][s] = k1[eo][s] [es] = k1[eo][s] k 1 + k2 + k1[s]