GENET270 Study Guide - Midterm Guide: Bacillus Subtilis, Antisense Rna, Relaxase

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The relaxosome is a complex made up of the proteins relaxase and helicase. Once the mating pair is formed, coupling proteins on the mpf site recruit the relaxosome, which then gets to work on the donor plasmid. It begins by making a nick in a single strand of the origin of the donor plasmid, and attaching to the free 5" end. The relaxosome is then translocated into the recipient cell while coupling proteins pump dna across the channel. The relaxosome then circularizes the conjugated dna via transesterification. Tra genes are genes found on the plasmid that encode the machinery necessary for conjugation. Upon the plasmid entering the cell, traj acts as a transcriptional activator for several other tra genes responsible for encoding the pilus and other machinery necessary for conjugation. Once the plasmid has been established, finp, an antisense mrna, bind to traj and inhibits it.

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