[BIOL 243] - Midterm Exam Guide - Comprehensive Notes for the exam (15 pages long!)

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Lab 1: (molymod kit exercise + dna extraction from strawberries) Dna (deoxyribonucleic acid): genetic blueprint for living organisms; stores genetic information. Formation of these bonds is done by dna polymerases prior to mitosis/meiosis. Dna s(cid:455)(cid:374)thesis p(cid:396)o(cid:272)eeds (cid:1009)" to (cid:1007)" al(cid:449)a(cid:455)s. (cid:1009)"-p of i(cid:374)(cid:272)o(cid:373)i(cid:374)g (cid:396)ea(cid:272)ts (cid:449)ith (cid:1007)"-oh of last in chain. Dna (cid:272)hai(cid:374) ulti(cid:373)atel(cid:455) has o(cid:374)e e(cid:374)d (cid:894)(cid:1009)"(cid:895) (cid:449)ith f(cid:396)ee p g(cid:396)oup, a(cid:374)d o(cid:374)e e(cid:374)d (cid:894)(cid:1007)"(cid:895) (cid:449)ith o(cid:374)e f(cid:396)ee oh g(cid:396)oup (cid:449)hi(cid:272)h is the o(cid:374)l(cid:455) place where a new nucleotide can be added. Al(cid:449)a(cid:455)s (cid:449)(cid:396)ite dna se(cid:395)ue(cid:374)(cid:272)es sta(cid:396)ti(cid:374)g f(cid:396)o(cid:373) the (cid:1009)" e(cid:374)d. (cid:862)phosphodiester backbone(cid:863) = phosphate-hydroxyl repeating framework to support dna molecule. Nitrogenous bases = where genetic information is stored. Template strand is passed on to the complementary strand with the help of dna polymerases stati(cid:374)g at the (cid:1007)" of te(cid:373)plate st(cid:396)a(cid:374)d (cid:894)(cid:1009)" of (cid:272)o(cid:373)ple(cid:373)e(cid:374)ta(cid:396)(cid:455)(cid:895); these two strands run antiparallel. A&t bind (2 h bonds) and c&g bind (3 h bonds).

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