BIOL 243 Study Guide - Midterm Guide: Interphase, Allele, Zygosity
Document Summary
Chromatin re-modelling: chromatin needs to be loosened to allow for transcription, activator binds the regulatory sequence, upstream of promotrer, which recruits swi-snf complex to siplace nucleosomes, saga (activator protein) recruits histome acetyltransferases (hats, acetylation. Meselson & stahl proved the semi conservative model. Mrna=messenger rna; translated ito proteins trna=transfer rna; transfers aas during translation. Rna pol attaches to dna and synthesis of rna begins. Pol ii with transcription factors bound to tata box (in the promoter); will still have basal level transcription if there"s no enhancer: elongation. New strand temporarily winds with dna, making a dna-rna hybrid: termination. Termination sequence causes rna dissociation from the template. Rna forms hairpin loop due to self complementary end sequence. Rho-helicase binds to terminator sequence and unwinds rna from dna. 5" g cap: cleavage & poly-a specific factor (cpsf) cleaves mrna after passingthe poly-a signal, poly-a tail added to 3" end, rat1 digvests what rna is still hanging loose from.
