BIOC 2580 Study Guide - Final Guide: Cytosol, Trypsin, Glucose

• School: University of Guelph
• Department: Biochemistry
• Course: BIOC 2580
• Professor: Peter Dawson

Document Summary

O linear chains of amino acids: linked by peptide bonds, each protein has a unique sequence of amino acids as well as defined size and properties. *study all amino acid cards and know polarity and structure* These side chain groups do not gain or lose h+ in aqueous solution, so they are uncharged -all 4 side chains act as good hydrogen bond donors or acceptors. Side chain gain h+ /become protonated at neutral ph. Lecture 3: amino acid analysis, helps determine protein structure, 2 processes involved. Low rf very polar amino acid-don"t move far. Competiton with a high ion concentration, which displaces the amino acid from the resin example na+ , displaces weakly bound amino acids, as na+ is increased more tightly bound amino acids are progressively displaced. Gives purple colour or yellow for proline. Colour intensity proportional to quantity of amino acid and can be measured: fluorescamine giving yellow fluorescence under uv light.