BIOC 4580 Study Guide - Midterm Guide: Immunofluorescence, Cysteine, Amphiphile

• School: University of Guelph
• Department: Biochemistry
• Course: BIOC 4580
• Professor: George Harauz

Document Summary

Bioinformatics of integral membrane proteins used to predict segments that are transmembrane, etc. Eukaryotic mostly -helical, bacteria most have - sheets. Hydrophobic tra(cid:374)s(cid:373)e(cid:373)(cid:271)ra(cid:374)e seg(cid:373)e(cid:374)ts (cid:894)eg. heli(cid:272)es(cid:895) ha(cid:448)e a positive free energy for going aqueous. Heli(cid:272)es are energetically favourable in a membrane information obtained from a hydrophobicity analysis: predicting transmembrane segments. Usually can predict transmembrane segments and orient relative to membrane and each other: sorting signals. Eukaryotic proteins often have signals such as nls (nuclear localization signal- run of basic residues) or sp (signal peptide) Prokaryotic proteins also have targeting signals for membranes: n-terminal segments of cytoplasmic proteins (tend to be hydrophobic) ** transmembrane helices do not necessarily span the entire lipid bilayer. Techniques for defining topology without a known crystal structure. A reporter gene or other identifiable motif (epitope, etc. ) is inserted into a particular splice site of your recombinant membrane protein. Gene fusion (prokaryotes) with a reporter gene eg. the gene encoding alkaline phosphatase (phoa fusions)