BIOC 4580 Study Guide - Midterm Guide: Lipid Bilayer, Cardiolipin, Electron Transport Chain

• School: University of Guelph
• Department: Biochemistry
• Course: BIOC 4580
• Professor: George Harauz

Document Summary

Class 17: mitochondria electron transport & proton pumping. Specialized mitochondrion eukaryotic membrane system derived originally from a prokaryotic membrane, and has a high protein-to-lipid ratio. In mitochondria, electron transport (via successive reduction-oxidation reactions and electron carriers) is the process that establishes a proton-motive force that is subsequently used to generate atp (cid:1006) t(cid:455)pes of (cid:862)e(cid:374)e(cid:396)g(cid:455) (cid:272)oupli(cid:374)g(cid:863) 2 membranes of varying permeability & protein content. Inner membrane 20-25% lipid, 60- 70% protein impermeable. Outer membrane 30-40% lipid, 60- 70% protein permeable. Pm has 30-50% lipid, 50-70% protein, 20% cholesterol. Imm has lots of cardiolipin & diacylglycerol (dag) Cardiolipin is enriched in & maintains fluidity of inner mitochondrial membranes. Allows membrane to undertake a specific shape (induces membrane curvature) Does(cid:374)"t fo(cid:396)(cid:373) (cid:271)ila(cid:455)e(cid:396) o(cid:374) its o(cid:449)(cid:374) (like pe, pa) Other lipids (pi, pc, ps) will form a bilayer. ** the mitochondrial inner membrane (imm) can form cubic phases in stressed cells. The mitochondria is a good indicator of cell health.