BIOC 4580 Study Guide - Midterm Guide: Cyclic Adenosine Monophosphate, B Cell, Immunoglobulin Superfamily

• School: University of Guelph
• Department: Biochemistry
• Course: BIOC 4580
• Professor: George Harauz

Document Summary

Bioc 4580 class 29: cell surface carbohydrates ii. The glycosylation of membrane proteins & lipids serves in intracellular transport & targetting, modulation of protein or lipid physical properties, and encoding biological information. Lectins recognise the information contained within carbohydrate structures. Toxins (cholera), viruses (influenza), bacteria (helicobacter pylori), and parasites (malaria) all use lectins to gain entry into cells. Nitrogen-fixing bacteria attach themselves to plant roots via lectins. Animal cells use lectins in cell:cell interactions during fertilization, embryonic differentiation, and inflammatory processes. Intracellular transport & targetting: modulation of protein physical properties, encoding biological information. Oligosaccharides have diverse roles in internal trafficking, and in recognition and adhesion at the cell surface. Often necessary but not essential for exocytosis. If block addition of n-linked oligosaccharides by tunicamycin, many membrane and/or secretory proteins stay stuck in rer & get degraded. Tunicamycin blocks the first pathways of adding sugars to the protein. Fibronectin can still carry out its function, but is degraded more quickly.