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BIOM 4070 Study Guide - Quiz Guide: Red Blood Cell, Goblet Cell, Hemoglobin

3 pages186 viewsFall 2016

Department
Biomedical Sciences
Course Code
BIOM 4070
Professor
Brenda L Coomber
Study Guide
Quiz

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Quiz 1 summary notes
First class and lab 1
Stains
H&E (hematoxylin and eosin)
Most common for paraffin
Most common for LM
Doesn’t differentiate carbohydrates, it will blend with cytoplasm
Turns lipid white
Pink: basic things (+ive), acidophilic/eosinophillic, mitochondria (lots of
transmembrane proteins), collagen, cytoskeleton
Purple: acidic things (-ive), basophilic, nucleic acid (DNA & RNA), RER (lots of
ribosomes, outweighs the protein)
Toluidine blue
Indicates relative protein levels (darker blue = more protein)
Most common for plastic
Transmission electron microscopy
Based on density (dense areas appear dark because more electrons reflect back)
Uses heavy metals
Higher resolution than LM because it has a shorter wavelength than light and
therefore higher energy/frequency, and can cut thinner slices
Scanning electron microscopy
Uses lighter metals (like gold), scatters reflected electrons
PAS (periodic acid-Schiff reagent)
Stains carbohydrates magenta (like glycogen, mucous…)
Alcian blue
Stains carbohydrates well
Tissue specific details
Pancreas: stain purple and pink with H&E (lots of RER to synthesize enzymes and lots of
stored dietary enzymes until needed), cells form acini (circular clusters) with a small
hole in the center (lumen  this is the apical end of the cell). These cells are polarized!
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