BIOM 4070 Quiz: Quiz 1 notes

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Department
Biomedical Sciences
Course Code
BIOM 4070
Professor
Brenda L Coomber

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Quiz 1 summary notes
First class and lab 1
Stains
H&E (hematoxylin and eosin)
Most common for paraffin
Most common for LM
Doesn’t differentiate carbohydrates, it will blend with cytoplasm
Turns lipid white
Pink: basic things (+ive), acidophilic/eosinophillic, mitochondria (lots of
transmembrane proteins), collagen, cytoskeleton
Purple: acidic things (-ive), basophilic, nucleic acid (DNA & RNA), RER (lots of
ribosomes, outweighs the protein)
Toluidine blue
Indicates relative protein levels (darker blue = more protein)
Most common for plastic
Transmission electron microscopy
Based on density (dense areas appear dark because more electrons reflect back)
Uses heavy metals
Higher resolution than LM because it has a shorter wavelength than light and
therefore higher energy/frequency, and can cut thinner slices
Scanning electron microscopy
Uses lighter metals (like gold), scatters reflected electrons
PAS (periodic acid-Schiff reagent)
Stains carbohydrates magenta (like glycogen, mucous…)
Alcian blue
Stains carbohydrates well
Tissue specific details
Pancreas: stain purple and pink with H&E (lots of RER to synthesize enzymes and lots of
stored dietary enzymes until needed), cells form acini (circular clusters) with a small
hole in the center (lumen  this is the apical end of the cell). These cells are polarized!
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Description
Quiz 1 summary notes First class and lab 1 Stains H&E (hematoxylin and eosin)  Most common for paraffin  Most common for LM  Doesn’t differentiate carbohydrates, it will blend with cytoplasm  Turns lipid white Pink: basic things (+ive), acidophilic/eosinophillic, mitochondria (lots of transmembrane proteins), collagen, cytoskeleton Purple: acidic things (-ive), basophilic, nucleic acid (DNA & RNA), RER (lots of ribosomes, outweighs the protein) Toluidine blue  Indicates relative protein levels (darker blue = more protein)  Most common for plastic Transmission electron microscopy  Based on density (dense areas appear dark because more electrons reflect back)  Uses heavy metals  Higher resolution than LM because it has a shorter wavelength than light and therefore higher energy/frequency, and can cut thinner slices Scanning electron microscopy  Uses lighter metals (like gold), scatters reflected electrons PAS (periodic acid-Schiff reagent)  Stains carbohydrates magenta (like glycogen, mucous…) Alcian blue  Stains carbohydrates well Tissue specific details Pancreas: stain purple and pink with H&E (lots of RER to synthesize enzymes and lots of stored dietary enzymes until needed), cells form acini (circular clusters) with a small hole in the center (lumen  this is the apical end of the cell). These cells are polarized! Nuclei are at basal end and proteins (digestive enzymes are stored in zymogen vesicles, stain purple in H&E or dark in EM) are at apical end (about to be secreted). Nuclei: may appear heterogenic (purple because of nucleic acid but pink because of proteins like histones) but overall tend to be mostly purple in H&E, dark in EM, light blue in toluene blue (less relative protein). Heterochromatin is dense and darker in EM, euchromatin is less dense and light in EM. Neurons: large neurons have prominent nuclei,
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