MCB 2050 Study Guide - Midterm Guide: Glucocorticoid, Heterochromatin, Autophagy

• School: University of Guelph
• Department: Molecular and Cellular Biology
• Course: MCB 2050
• Professor: D Mosser

Document Summary

Topic 4 regulation of gene expression in eukaryotes. 2: add antibodies designed specific for a tf, this antibody chromatin complex will precipitate (hence the name, tf and antibodies are removed, dna is now purified, purified dna can be sequenced directly, or amplified by pcr to use as a probe to perform chip chip assay, gel electrophoresis is just for detection whether we successfully pulled down the sequence. Inhibit translation by blocking initiation/elongation mirna synthesis: large pri mirna transcribed from the dna, dorsha cuts the tails/ends of the pro mirna, producing pre mirna, pre mirna is exported out of the nucleus by exportin 5, dicer cuts the loop of the pre mirna in the cytosol, leaving a dsrna, this dsrna associates with argonaute 2 protein , forming rna induced silencing, dsrna is unwind, the passenger strand of the dsrna is degraded, the guide strand base pairs with a specific mrna sequence to silence the mrna complex (risc)