MCB 2050 Study Guide - Final Guide: Genetically Modified Crops, Genetically Modified Organism, Gene Therapy

103 views6 pages

Document Summary

Somatic gene therapy gene correction in non-germline cells (non-heritable) Transgene: gene introduced into an organism delivering transgenes by fusing with cell membranes. Infect bone marrow stem cells with il2r c+ retroviral vector, integrated into the chromosomes of bone marrow stem cells: grow cells in culture, verify the expression of the human il2r c+ transgene. Infuse stem cells that are expressing the il2r c+ transgene into the patient: synthesis of il2r c protein restores immune function in white blood cells after the infusion of stem cells carrying il2r c+ transgene. Recombinant protein production: eukaryotic proteins (pharmaceutical values) can be produced economically in genetically engineered bacteria or yeast, example: production of human growth hormone (hgh) in e. coli. Use expression plasmid (that can express a protein needs promoter, binding sequence, essential stuff for expressing gene) Piece together with signaling dna molecule, clone into plasmid, put into bacteria strain, grow strain, extract the protein.