MBG 4270 Study Guide - Final Guide: General Products, Transferase, Proliferating Cell Nuclear Antigen
13 Apr 2017
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Non-homologous-end joining: genetic exchange between sequences sharing little or no similarity. Site-specific recombination: recombination between two defined sequence elements (small target sequences) Interrupt gene coding sequence, disrupt linkage between coding and regulatory sequences, alter chromosome arrangement, influence replication, dna packaging and chromosome segregation, result in mutagenic dna arrangements, substrate for exonuclease, loss of genetic information. Endogenous sources oxidizing agents in cells; topoisomerase i activity. Mitomycin c potent dna cross linking agent, arrests replication fork = dna breaks. Can be fixed properly or sometimes joined aberrantly by recombination in cells. Hr: exchange of genetic information between similar/identical dna sequences initiated by nicks or dsb. In eukaryotes: homologous chromosomes in meiosis (required for chromosome dysjunction, mitotically dividing somatic cells dna repair mechanism in late s/g2 phase of cell cycle; restarts stalled replication forks; recombination between repeats. In prokaryotes: between repeated sequences; restarting stalled replication forks; dna transformation and conjugation.
