MCB 2050 Study Guide - Midterm Guide: Southern Blot, Northern Blot, Dna Microarray
19 Apr 2013
School
Department
Course
Professor
Document Summary
Southern blotting can be used to determine if a genomic dna clone has a particular sts. Ori is a common feature of all cloning vectors. Dna ligase joins the ends of two dna fragments. Dna polymerase is required in sanger"s dna sequencing reaction. Hat complex is involved in chromosome remodeling. Dna microarray technology for transcription profiling hybridize fluorescently labeled cdna sample to dna probes on the array. Luman is activated by proteolytic cleavage and regulated by post-translational regulation. Mirna"s usually result in the repression of translation of the target mrna. According to the human genome project the percent of human genome containing exons that code for proteins is 1% Transcription of a gene, splicing of an rna molecule, migration of an mrna molecule into the cytoplasm, polypeptide synthesis, degradation of an mrna molecule. Zn finger domains are dna binding domains. Snp"s on a chromosome segment are inherited together and define genetic unit is called haplotype.
