PSYC 3470 Study Guide - Final Guide: Mannose, Lysosome, Peptide
10 Apr 2019
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Before attending tutorial 7, label the diagram and define the terms listed below. Note you will need some of this information in order to answer the marked question 3. The proper trafficking of lysosomal hydrolylase (lh) enzymes to the lysosome requires the presence of one or more mannose 6-phosphate (m6p) residues in their n-linked oligosaccharide chain(s). The phosphorylation of the mannose sugars on lysosomal-destined proteins is mediated in part by the cis-golgi enzyme n-acetylglucosamine phosphotransferase (glcnac-pt). M6p then serves as a signal patch that is recognized by the m6p receptor, a membrane protein found in the trans-golgi network, which also mediates the subsequent packaging of the m6p-bearing lysosomal proteins into clathrin-coated transport vesicles. You construct four plasmids expressing gfp-tagged wild-type or three different mutant version of an lh enzyme in order to study (via confocal laser-scanning fluorescence microscopy) its intracellular trafficking.
