TOX 4590 Study Guide - Quiz Guide: Bioassay, Standard Deviation, Absorbance
Document Summary
Bioassay #1 - acute toxicity testing using biological systems. Average blank = (0. 047 + 0. 055 + 0. 46 + 0. 46 + 0. 46 + 0. 047 + 0. 048 + 0. 048)/8. *calculated using empty wells since blank was contaminated. Observations: (containing the blank), changed to a dark purple. Column 3 and 4 showed a colour gradient starting with clear solution in 3a and ending with a dark purple in 4h. Sample 1 developed a deep purple colour in all wells, sample 2 was mostly clear in all wells with a faint hue of pink in some, and sample. The negative control produced a dark purple colour. A colour change was immediately observed upon addition of chromogen to the plate. Plat was set up to include a blank (1), positive control gradient (3+4), sample 1 (6), sample 2 (8), sample 3 (10), and a negative control (12). Tf = [1 (od* treated wells/average od of negative control wells)]