BGEN 3020 Study Guide - Fall 2018, Comprehensive Midterm Notes - Gene, Dna, Genome

• School: University of Manitoba
• Department: Biochem. and Medical Genetics
• Course: BGEN 3020
• Professor: Merz

Document Summary

Complete dna sequence of a cell or an organism. How many different genomes are in a typical human cell: there are 2 genomes in most cells, mitochondrial (mt) genome, nuclear genome (what most people think of when you say genome) A red blood cell: has no dna content (i. e. no genome) 20 000+ protein-encoding genes: note: protein encoding genes here is important (there are also many genes that do no encode proteins, people expected to find at least 100,000+ genes in the human genomes. Used old methods of cloning, mapping and sequencing (sanger: published in 2001, computer predictions (orfs, splice sites, etc. ) verified with cdna sequencing (ests etc. ) i. e. computers make the predictions, and we verify them experimentally with cdna sequencing. Human 3000 mb, 20000 genes, 46 chromosomes. C. elegans 100 mb, 20000 genes, 12 chromosomes. Arabidopsis 140 mb, 27000 genes, 42 chromosomes. Wheat 16800 mb, 95000 genes, 42 chromosomes: wheat has the largest genome out of all the ones listed.