CHEM 2360 Study Guide - Final Guide: Protonation, Sodium Hydroxide, N-Terminus

• School: University of Manitoba
• Department: Chemistry
• Course: CHEM 2360
• Professor: Ellert R.Nichols

Document Summary

Experiment 2: buffers, buffer capacity, and amino acid titrations. Titration of amino acids: amino acids have >1 ionizable group, the carboxy terminus (pka ~1. 9-2. 4, the amino terminus (pka ~8. 8-10. 9, some have ionizable r groups, for each ionizable group, the amino acid will have a region of buffering. 1 ph unit of the pka for those ionizable groups. Accurate ph measurements cannot be accomplished with a ph meter unless the meter has been calibrated against standardized buffers. The ph meter will compare all other ph readings to these standard buffers. Bring the final volume to 1 l: method 3: weigh out enough a- to give the correct concentration, dissolve in h2o and then add hcl to generate the required amount of weak acid. Part c: buffering capacity: add ph to buffer solutions with varying concentrations of phosphate buffer, observed: lower net ph change for higher concentrations of phosphate buffer.