MBIO 2360 Study Guide - Quiz Guide: Vitamin C, Ferrocyanide, Reduction Potential

• School: University of Manitoba
• Department: Microbiology
• Course: MBIO 2360
• Professor: Sean A.Mc Kenna

Document Summary

0. 1 e c n a b r o s b. The absorption spectrum of fully oxidized cytochrome c (a) and reduced cytochrome c (b): 2. 7 ml of 0. 4 mg/ml cytochrome c solution from equine heart in phosphate buffer mix with. 300 l of 60. 0 mm ferricyanide in phosphate buffer in cuvette#1: 2. 7 ml of 0. 4 mg/ml cytochrome c solution from equine heart in phosphate buffer mix with. The difference spectrum for change in absorbance between the fully reduced (a) and fully oxidized forms of cytochrome c (b): 2. 7 ml of 0. 4 mg/ml cytochrome c solution from equine heart in phosphate buffer mix with. 300 l of 60. 0 mm ferrocyanide in phosphate buffer in cuvette#2. Difference spectral methods, to be described, also offer the possibility of dealing experimentally with questions concerning the involvement and location of natural and artificial chromophoric groups in enzyme-substrate and protein-protein interactions. Difference spectra can be used to obtain information on denaturation and proteolysis of proteins.