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Study Guide

MBIO 3280- Final Exam Guide - Comprehensive Notes for the exam ( 84 pages long!)


Department
Microbiology
Course Code
MBIO 3280
Professor
Vladimir Yurkov
Study Guide
Final

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UofM
MBIO 3280
FINAL EXAM
STUDY GUIDE

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MBIO 3280 Lecture 1
Office #422 Buller
Office hours: when door is open
Number 474 9045
Email: vyurkov@umanitoba.ca
Email for appointment
- Does not check emails after 5
- Start email with name and student number
Textbook
- Brock biology of microorganisms
- Tested only on lecture notes use textbook for reference only
Course Evaluation
- Midterm = 20%
o Date not established (will vote on dates)
o In class
o No questions during midterm due to size of room
o Combination exam of multiple choice, short answer, truth or false (with
explanation if false)
- Lab = 20%
- Final exam = 60%
o cumulative
Lab
- Labs with Chris Rathgeber
- Room 312 start sept 11th
- Room 419
- Chris.rathgeber@umanitoba.ca
Course Outline
- Introduction/overview
- History of Microbial Ecology and Microbial Communities Study
- Environment and Physiology
- Biogeochemical Cycling of Elements: Carbon, Nitrogen, Sulfur and Phosphate/Iron
- Microbial Habitats Soil, Ocean, Lakes, Extreme Environments
- Microbial Associations and Interactions: Microbes with other microbes, plants, insects
and animals
- Applied microbial ecology
- Methods in microbial ecology
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MBIO 3280 Lecture 2
The importance of microbes in nature
- Widespread distribution
o Easily dispersed
Can be transferred by air, as well as spores
Humans, and physical objects
o Diverse
Phenotypical, and genotypic
o Present for > 3 billion years
- Abundance
o Small size but large numbers
o Estimated that 1 hectare of fertile soil 15 cm deep has 1000kg of microbes
(mostly bacteria and fungi)
- Adaptable
o Fast growth rates allow reproduction to large numbers
o Fast adaptation to environment through molecular regulation
- Survival
o Long times with minimal energy requirements
Some go into dormant forms
o Extreme environments
Hydrothermal vents
Sea ice
Desert
- Microbial population
o Groups of cells derived from a single parent cell via cell division
- Habitat
o Physical location (ie. Environment) in which microbial population lives
- Microbial Community
o Interacting multiple populations
o Diverse and abundant
Controlled by resources (foods) and conditions (ie. Abiotic factors)
- Diversity of metabolism (exclusive to bacteria)
o Anaerobic respiration
Sulphate respiration
Metal oxides
Nitrate
o Chemolithotrophy
Mode of energy generation only involving inorganic materials
o Nitrogen fixation
Fix nitrogen from atmosphere
o Biodegradation of xenobiotic compounds
Not produced by organisms not biotic
- Mixed populations work together
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